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Hydrostatic pressures (HP) less then 30-40 MPa are often considered mild, and their impact on petroleum biodegradation seldom considered. However, the frequent use of nutrient-rich media in lab-scale high-pressure reactors may exaggerate HP importance by resulting in a strong growth stimulation as compared to oligotrophic marine environments. Here, we tested coastal seawater microbial communities, presumably enriched in pressure-sensitive microorganisms. Limiting environmental conditions for growth were applied (i.e. low temperature [5°C

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