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Exosomes are membrane-covered nanovesicles that will transport microRNAs (miRNAs) and other molecular indicators between cells. In other types, serum exosome-derived miRNAs can serve as great biomarkers of diseases and different physiological states, including maternity condition. We hypothesized that circulating exosome-derived miRNAs might be used to differentiate the pregnancy status as soon as several times after insemination in pigs. To test this theory, we arbitra