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ELISA ended up being used to examine the production of IGF-1. The proliferative effect of L-PRF on SCs had been considered with CCK-8 assay. The aftereffect of L-PRF in the mRNA and necessary protein expression of SC neurotrophic aspects had been reviewed by RT-qPCR and ELISA. CCK-8 assay and RT-qPCR were used to look for the required focus plus the activity time of PG-LPS before the anti-inflammatory effect of L-PRF had been determined by measuring the changes in IL-1β, IL-6,
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