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All EV subpopulations were then analysed in depth making use of electron microscopy, Bioanalyzer®, nanoparticle monitoring evaluation, and quantitative mass spectrometry evaluation. Subpopulations of EVs with distinct size, morphology, and RNA and protein cargo could be separated through the metastatic melanoma tissue. LD EVs showed an RNA profile using the presence of 18S and 28S ribosomal subunits. In comparison, HD EVs had RNA profiles with little or no peaks f
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