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As one step forward, a lateral flow immunoassay was created for onsite evaluating analysis of spirotetramat in wine. This assay was successfully validated according to Regulation 519/2014/EU for semi-quantitative practices at levels in line with the legal levels of spirotetramat and spirotetramat-enol in red grapes, with a satisfactory false suspect price below 2%.Top-down and bottom-up forces determine ecosystem function and characteristics. Fisheries as a top-down force