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This study had the dual objective of both the qualitative and quantitative assessment of plastic mixtures sampled in 5 different sites located along the Lambro River (northern Italy), and the contemporarily determination of the ecotoxicological effects of the same mixtures sampled, through 21-day laboratory exposures of the freshwater bivalve Dreissena polymorpha. The monitoring survey was carried out by a Fourier Transform Infrared Microscope System, while the ecotoxicological assessment was performed by the mussel mortality, a biomark