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The current study aimed to verify the changes in performance, physiological and biomechanical variables throughout a season in master swimmers. Twenty-three master swimmers (34.9 ± 7.4 years) were assessed three times during a season (December M , March M , June M ), in indoor 25 m swimming pools. An incremental 5 × 200 m test was used to evaluate the speed at 4 mmol·L of blood lactate concentration (sLT), maximal oxygen uptake ( ), peak blood lactate ([La-]peak) after the test, stroke frequency ( ), stroke length ( ), stroke index ( )