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The overall kinds of programs and fields of use that have appeared for affinity chromatography will also be considered. A survey of the literature is used to recognize major trends during these subjects and essential regions of usage for affinity chromatography in the separation, analysis, or characterization of chemicals and biochemicals.A brand new HPLC method was developed when it comes to enantio-separation and chiral recognition method of quinolones (lomefloxacine, ofloxacine