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To understand the pathogenesis in rat corneal endothelial cells (RCECs) induced by murine cytomegalovirus infection . , cultured RCECs were infected with murine cytomegalovirus strain K181-eGFP (MCMV-eGFP). , experimental rats received intracameral injection of MCMV-eGFP. Replicating viruses and morphology change of RCECs were evaluated at several time points. , RCECs became necrosis at 6hpi. MCMV-eGFP began replicating at 12hpi. , the inflammatory reactions appeared at 12hpi, peaked at 72hpi and gradually subsided. Replicating MCMV-eG