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Sealant integrity was compromised in more than 50% of the ROIs already after 3months for both sealants. Patients treated with fixed orthodontic surface sealants lost the integrity of the protective layer in more than 50% of cases after 3months, and the layer thickness of the sealants was significantly reduced after 3-6months. The protective effect against demineralization lesions of orthodontic sealants in patients treated with fixed appliances appears to be limited in time. Further preventive measures should be investigated. ClinicalTr