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Although most ARGs except for quinolones were widely distributed in domestic wastewater discharge and effluent from WWTPs, macrolide resistance genes (ermB and msrA) were detected mainly from oysters in Kamak Bay. This study will aid in tracking the sources of antibiotic contamination in shellfish to determine the correlation between shellfish and inland pollution sources.Recently, reservoirs in southern China are witnessing incidents involving black water, which are harmful to the aquatic ecosystem. This study unravels the cause of the