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This induced the activation of PBMCs, giving rise to an immune response, which is followed by the development of the inflammatory response. The results from this study could be used as the basis for the development of novel vaccine candidates against M. bovis.Correction of the chemical composition effect for soil samples in gamma spectrometry is very important to perform at low emission energy ( less then 100 keV). This study presents an intensive Monte Carlo simulation using Geant4 code of CERN which investigates several items in this subject. First, it is