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These values were lower than those for the control agent ningnanmycin (0.19 μM) and ribavirin (6.54 μM), which suggested that the compounds had a solid binding effect with ToCVCP. Quantitative real time PCR (qRT-PCR) ended up being made use of to judge the part of substances B13 and B23 within the gene legislation of ToCVCP. Both substances considerably decreased the expression degree of ToCVCP gene in Nicotiana benthamiana with reduction values of 88% and 83%, which were a lot bet