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Screening suitable strains with high temperature adaptability is of great importance for reducing the cost of temperature control in microalgae cultivation especially in summer. To obtain high temperature tolerant diatoms, water samples were collected in summer from 7 different regions of China across the Northeast, North and East. A total of 731 water samples was collected and from them 131 diatom strains were isolated and identified based on the 18S rRNA sequences. Forty-nine strains out of the 131 diatoms could survive at 30 °C, and