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We suggest that CRF therapy is capable of inducing thermogenic adipocytes in lean mice. The role of oxidative stress during cryoprotectant treatment has received little attention. OBJECTİVE To assess the effects of different cryoprotectants and discover relationships between cryodamage and oxidative stress parameters on Awassi ram sperm. The sperm samples diluted with Salamon's tris-citrate (TRIS) containing 20% centrifuged egg yolk and 0.5, 1.0 or 1.5 M Glycerol (Gly), methanol (M), 2-methoxyethanol (2-ME), dimethylacetamide (DMA) and