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Our novel protocol of tissue culture may be applicable for eye banks to optimize corneal grafting. © 2020 The Authors. Journal of Cellular and Molecular Medicine published by Foundation for Cellular and Molecular Medicine and John Wiley Sons Ltd.Small open reading frames (sORFs) which are translate to small peptides (100 amino acids or fewer in length) have been always excluded from genome annotations. In recent years, more and more biologically significant sORFs have been discovered to encode functional peptides or play regulat