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Cxxc1 deletion also led to a significant decrease of H3K4me3 enrichment at DMC1-binding sites, which might compromise DSB generation. Taken together, our results show that CXXC1 is essential for proper meiotic crossover formation in mice and suggest that CXXC1-mediated H3K4me3 plays an essential role in meiotic prophase of spermatogenesis and oogenesis. © 2020. Published by The Company of Biologists Ltd.Myosin heavy chain-embryonic (MyHC-emb) is a skeletal muscle specific contractile protein expressed during muscle development. Mutations