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This study quantified the uncertainty sources of the PN systems (i) due to the technical requirements and the calibrations, (ii) due to the unknown particle sizes during measurement, (iii) due to particle losses from the vehicle to the PN systems at the tailpipe or the dilution tunnel, (iv) other parameters needed for the calculation of the emissions, non-related to the PN systems, e.g. flow and distance. The expanded uncertainty of the 23 nm laboratory systems sampling from the dilution tunnel was estimated to be 32%, with 18% origina