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The results suggested that the synthesized MgO-biochar with enough MgO active site dispersed on a higher external surface can be used as a potential adsorbent for phosphate removal and recovery from aqueous solution. Biphenyl 2,3-dioxygenase (BphA), a Rieske-type and first enzyme in the aerobic degradation process, plays a key role in the metabolizing process of biphenyl/polychlorinated biphenyl aromatic pollutants in the environment. To understand the catalytic mechanism of biphenyl 2,3-dioxygenase, the conversions leading to the cis-dio