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Instance history, autopsy, and toxicological examination of ante- and post-mortem collected materials are explained. Blood and urine collected through the patient ante-mortem and other a few biological materials (particularly bloodstream through the top and reduced limb, blood from the correct and left ventricle, pericardial liquid, vitreous humour, liver, renal, and spleen) were gathered post-mortem during autopsy. Liquid-liquid removal procedure followed by high-performance