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The capability of FUS+GEM to control main cyst outgrowth had been mildly improved by either neoadjuvant or adjuvant treatment with anti-PD-1. Thermally ablative FUS in combination with GEM limits main tumor outgrowth, improves survival and improves immunogenicity in a murine metastatic TNBC design. This treatment strategy claims a novel selection for potentiating the role of FUS in immunotherapy of metastatic TNBC and is worth future clinical assessment. The immunological microenvironment of prim