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Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging is an effective tool for investigating the distribution of molecules. However, cryosections are made from non-fixed tissues, causing difficulties in preparing sections from fragile, high-water content tissues such as those from tadpoles. Here, we introduce a new method for preparing cryosections using an adhesive tape followed by transfer onto glass slides for MALDI-MS imaging. Signals obtained from the transferred sections were higher than those from other se