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Polymicrobial biofilms, supplemented with δ-aminolevulinic acid, were investigated likewise. Results an overall total of 28/32 bacteria, 1/4 yeast species and polymicrobial biofilms produced red fluorescence, in arrangement along with their understood porphyrin production capabilities. Conclusion These results identify common wound pathogens with the capacity of making porphyrin-specific fluorescence and help clinical observations making use of fluorescence imaging to det