https://cellbasedassayblog.com..../security-along-with
Herein, we aimed to examine very early and late systems of MTX-induced cardiotoxicity using murine HL-1 cardiomyocytes. Cells had been confronted with MTX (0.1, 1 or 10 µM) during brief (2, 4, 6, or 12 h) or longer incubation periods (24 or 48 h). At previous time things, (6 and 12 h) cytotoxicity was already observed for 1 and 10 µM MTX. Proteomic analysis of complete protein extracts found 14 proteins with higher appearance and 26 with reduced phrase when you look at the
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