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These residues, together with Arg8 (which is a hot-spot residue in the interaction with GS), had a restricted mobility in the presence of GS. The C-terminal region, which appeared more compact in our MD simulations of isolated IF7, seemed to be involved in non-native contacts with GS that help in the binding between the two macromolecules. In this study, high concentration of H2S (i.e., 5000 ppmv) in biogas was effectively removed by a slightly alkaline biotricking filter (BTF) with Polypropylene rings as packing material and oxygen from a
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