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The present research showed that verification of infection, correspondingly transmission can not be predicated on an individual test. Only combined serological antibody make sure real time PCR exams of delivery material, milk, feces and vaginal mucus can lead to a conclusive diagnosis. In addition, the examination using modified ZN staining according to Stamp is less sensitive and specific compared to real time PCR assessment. The subscription of damaging events after the utilization of immunologic