https://www.selleckchem.com/pr....oducts/gsk2193874.ht
We describe a workflow for preprocessing of single-cell RNA-sequencing data that balances efficiency and accuracy. Our workflow is based on the kallisto and bustools programs, and is near optimal in speed with a constant memory requirement providing scalability for arbitrarily large datasets. The workflow is modular, and we demonstrate its flexibility by showing how it can be used for RNA velocity analyses.Functional imaging using fluorescent indicators has revolutionized biology, but additional sensor scaffolds are needed to access
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