https://www.selleckchem.com/pr....oducts/gs-441524.htm
8492, p 0.0001). The SARS-CoV-2 detection rate by direct RT-qPCR was 95.8 % for Ct values 35. All negative samples were characterized by low viral loads (Ct 35) and/or long storage times before sample processing. Direct RT-qPCR is a suitable alternative to classical RNA RT-qPCR, provided that only fresh samples (storage 1 week) are used. RNA extraction should be considered if samples have longer storage times or if PCR inhibition is observed. In summary, this protocol is fast, inexpensive and suitable for all respirat
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