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Ecological assessment was performed in different thermal springs located in the Ethiopian Rift Valley area Gergedi, Sodere, Halaba, and Gara. We assessed the habitat conditions, physicochemical characteristics, and macroinvertebrate composition at 12 sampling sites in four thermal spring systems. The physicochemical properties of the water samples varied among the sampling stations. Temperature ranged from 38.5 to 90°C among the 12 sites. Dissolved oxygen, which is essential to aquatic ecosystem normal functioning, was not detected at