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The neonicotinoids are globally used insecticides, which have been shown to cause negative impacts on birds. The current study aimed to evaluate the distribution of the neonicotinoid imidacloprid (IMI) in the tissues of a songbird and identify related physiological effects. Adults of the grayish baywing (Agelaioides baduis) were administered with a single dose of 35 mg IMI/kg, and the IMI concentration was evaluated in liver, kidney and plasma at 4, 12, 24, and 48 h after dosing. At the same time points, effects on hematological, genetic