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By mutagenesis of this Aft1-binding internet sites within RNR1 promoter, we conclude that RNR1 activation by metal depletion is essential for Rnr1 protein and deoxyribonucleotide synthesis. Remarkably, Aft1 also triggers the phrase of IXR1 upon iron scarcity through an iron-responsive factor positioned within its promoter. These results offer a novel procedure for the direct activation of ribonucleoti