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Based on molecular analyses, the transgenicity of 21.42% of the regenerated vitroplants was confirmed. The developed regeneration and transformation procedures of the elite 'Maltese half-blood' variety can be used for orchard renewal as well as for functional studies and genome editing purposes to develop new cultivars with the desired genetic traits. © King Abdulaziz City for Science and Technology 2020.The present investigation aimed to look at the effects of biotic and abiotic elicitors during Soybean seed development and cell suspen