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The applicability was verified by investigating 5fC content change in genomic DNA of rice tissues after incubated with heavy metals. Moreover, the inhibited influence of hydrochar towards heavy metals was also assessed.A preparation of colloidal activated carbon (CAC) for phenol remediation from groundwater was introduced. The CAC prepared by a simple pulverization technique was an excellent metal-free catalyst for persulfate (PS) activation due to high contact surface area. The removal efficiency of phenol in the PS/CAC system (~100%)