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Compared to alcohol group, puerarin and PUE-NP increased the disappearance latency time of righting reflex, and also the data recovery time of righting response ended up being dramatically shortened. Water maze results showed that Puerarin and PUE-NP had inhibitory influence on impaired memory. HPLC results revealed that PUE-NP reached its peak in mice after 1 h, as well as the content percentage was twice that of puerarin planning alone, and also the distribution period of puerarin focus in