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The nitrate quantity was higher than the permissible limit, in the water distribution network of Bushehr, Gilan and Mazandaran Provinces. Talesh, Ardabil, Hashtgerd, Divandareh, and Kerman cities had high nitrate levels in more than 50% of wells. Using nitrogen fertilizers and lack of a wastewater treatment system were the main reasons for the presence of nitrate and nitrite. Using nitrogen fertilizers and lack of a wastewater treatment system were the main reasons for the presence of nitrate and nitrite. Type 2 Diabetes Mellitus (T2DM