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In addition, overexpression of these genes of interest had no effect on cell proliferation and viability. Taken together, the results indicate that these engineered cells could be used as a cell-based system to propagate influenza virus efficiently in the absence of trypsin. Further studies on influenza virus interactions with chicken cell host factors could be studied without the effect of trypsin on cells. Taken together, the results indicate that these engineered cells could be used as a cell-based system to propagate influenza viru