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CKD ended up being induced in rats via adenine orThy1 antibody shot. Phosphate uptake by abdominal brush edge membrane vesicles (BBMV) and also the messenger RNA (mRNA) expression of NaPi-IIb, PiT-1 and PiT-2 had been analyzed. The protein expression degree of NaPi-IIb was calculated by mass spectrometry (example. fluid chromatography combination mass spectrometry). In regular rats, phosphate uptake into BBMV contained a single saturable component a
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