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Colorimetric aptasensors have been intensively studied for the ochratoxin A (OTA) detection, but they mostly exhibit just one-color change, resulting in poor visual resolution and limited use for semi-quantitative analysis. Thus, we designed a high-resolution colorimetric assay on the basis of aptamer structural switching and enzyme-induced metallization of gold nanorods (AuNRs). DNA-alkaline phosphatase (ALP)-immobilized magnetic beads were prepared. The aptamer bounded to OTA to form G-quadruplexes, releasing ALP-labelled complementary