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9% variance; P less then 0.001); whereas clothianidin, acetamiprid, and thiacloprid were mainly influenced by soil depths (accounting for 66.9-85.2% variance; P less then 0.05). Redundancy analyzes further indicated that the enhanced accumulation of neonicotinoids was mainly correlated with the increase of soil organic carbon (SOC) content and soil porosity, and the reduction of bulk density in the profiles of citrus orchards with increasing cultivation age. This study highlights the finding that we should give more concerns about the