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rch. Culture is still the gold standard for the detection of genital mycoplasma which could cause urogenital infections in humans. Mycoplasma IST2 is a commercial kit widely used for the detection of and species. Its accuracy was partially impaired because clinical specimens are usually mixed with purulent or transparent mucus. We aimed to solve this problem through sample homogenization by -acetylcysteine (NAC) treatment. Twenty-two endocervical swab samples were collected from 22 female patients with suspected mycoplasma infection, whi