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We also demonstrated the utility of the proposed method by applying it to digital PCR and digital microbial assays.In this work, a core-shell structured upconversion nanoparticles@zeolitic imidazolate frameworks (ZIF-8) fluorescent nanoprobe (NaErF4Tm@SiO2@ZIF-8) has been designed for the detection of gallic acid (GA). The mechanism is according to the 3, 3', 5, 5'-tetramethylbenzidine (TM can be oxidized to oxidized TMB (oxTM by Ag+. Under 980 nm laser excitation, NaErF4Tm@SiO2@ZIF-8 can emit red light at 652 nm, which have a good o
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