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chinensis Benth was closely related to Ocimum basilicum, indicating that there was a certain correlation between the CUB of the chloroplast gene and the genetic relationship of plant species. Overall, the study on the CUB of chloroplast genome laid a basis for genetic modification and phylogenetic research of M. chinensis Benth chloroplast genome.The adverse effect of tetracycline antibiotics on microbial activity is one of the serious risks for the biologic wastewater treatment process. The microbial fuel cells (MFCs) are a promising t