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Moreover, the high ratio of both α-helix and intermolecular hydrogen bond at an acid condition could enhance the gel-strength of EPS, which provide AGS the resistance ability against external disturbance. This study revealed the mechanism of the interactions in EPS adhesion process with the variation of pH and provided useful information for a better understanding of the stability of the AGS.Biofouling impedes the performance of anaerobic membrane bioreactors (AnMBR). Two reactors, one as an up-flow attachment-growth AnMBR (UA-AnMBR) conf