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e., serum and urine) we show that it is possible to link the misclassified samples to specific metabolic imbalances, such as carotenoid levels, or variations in the creatinine concentration.In this work, a nitrogen-doped carbon dots (CDs) was successfully synthesized by hydrothermal synthesis of polyethylenimine (PEI) and citric acid. The as-prepared CDs suffered from aggregation-caused quenching (ACQ) with a high concentration, but after adding adenosine triphosphate (ATP), the CDs aggregated. The generation of aggregates caused the ro