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In this experiment, natural nontoxic preservative ε-polylysine (ε-PL) was used as a natural preservative in pork jerky. The pork jerky samples with ε-PL (experimental group) and without ε-PL (blank group) were stored at the 27 and 37℃. Then, the number of microorganisms, total volatile basic nitrogen (TVB-N), pH, and water activity (Aw) of each group were tested to test the antiseptic effect of ε-PL. The results showed that due to the Staphylococcus aureus was detected, the storage period of the blank group at 27 and 37°C was 15 and 9 d