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In the case of fetal free DNA concentration less than 4%, the success rate of obtaining effective NIPT results by re-sampling and re-detection increases because of the increase of gestational age, but may delay the analysis for fetal aneuploidies. Therefore, personalized estimation should always be made relating to gestational age and medical indications. It's advocated that expecting mothers should select unpleasant prenatal analysis once they failed within the retest.Wit