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However, few practices being reported when it comes to multiple dedication of reduced kinds and their oxidized types of thiols in plasma. Techniques we developed a hollow fiber centrifugal ultrafiltration (HFCF-UF) means for test planning and validated a high-performance fluid chromatography combination size spectrometry (HPLC-MS/MS) way to determine two reduced kinds of thiols-homocysteine (Hcy), cysteine (Cys) amounts and their particular oxidized substances, homoan Cys/Cy