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Parasites had been used at regular periods across an extended isoleucine starvation time course over the cellular period utilizing flow cytometry. RESULTS These experiments disclosed that isoleucine-deprived parasites never exit the cell cycle, but alternatively constantly grow at a markedly paid off pace. Moreover, sluggish development does occur as long as isoleucine is taken away ahead of the onset of schizogony. After S-phase commenced, the parasite is insensitive to isoleucine depletion