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Furthermore, we discovered that the sheer number of viable hepatocytes after cryopreservation were 70% greater beneath the 2.6μM trehalose-supplemented conditions than under the dimethyl sulfoxide-supplemented problems. More over, non-cryopreserved cells and cells cryopreserved with trehalose showed similar intracellular dehydrogenase activity.We showed that trehalose at low levels (2.6 μM) improved dramatically viability and liver purpose of hepatocyte after cryopreservation.Conventional per