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ctice with comparable utility to the current standard DS, whereby providing a shorter and more comfortable perimetry experience. The purpose of this study was to compare conventional diagnostic culture (CDC) to 16S ribosomal RNA polymerase chain reaction (PCR) analysis for diagnosing bacterial keratitis. Samples collected from 100 consecutive patients presenting to the Royal Liverpool University Hospital with bacterial keratitis were processed using CDC and 16S PCR analysis. The overall detection rate using both methods was 36%. Of these,